Fig. 8: Accelerated tumor growth in sympathectomized KIC mice.

a Outline of the experiment. b, c Representative pictures of tumoral pancreata from 6.5-week-old KIC mice treated with AA (b) or 6-OHDA (c). d Scattered dot plot of pancreatic weight from 6.5-week-old KIC mice treated with AA (KIC^AA, n = 9) or 6-OHDA (KIC^6-OHDA, n = 13). P = 0.0348 (unpaired t test with Welch’s correction). e, f Bright-field images of HPS-stained pancreatic sections from 6.5-week-old KIC mice treated with AA or 6-OHDA. Images are representative of 3 mice analyzed per treatment group. g Pie charts showing the percentage of surface covered by asymptomatic tissue (Asympt), ADM, PanIN, and PDAC in pancreatic sections from 6.5-week-old KIC mice treated with AA (n = 3 mice, 12 sections) or 6-OHDA (n = 3 mice, 12 sections). h–o Representative images of immunohistochemistry for Ki-67 (h, i), Masson’s trichrome staining (j, k), immunofluorescence for PECAM (l, m), and pimonidazole staining (n, o) in PDAC sections from 6.5-week-old KIC mice treated with AA or 6-OHDA. p–s Scattered dot plots of the densities of Ki-67⁺ cells (p), collagen (q), PECAM⁺ vessels (r), and pimonidazole⁺ hypoxic areas (s) in PDAC sections of 6.5-week-old KIC mice treated with AA or 6-OHDA. p P = 0.0145, unpaired t test with Welch’s correction (KIC^AA, n = 3 mice, 19 images; KIC^6-OHDA, n = 3 mice, 22 images). q P = 0.0085, unpaired t test with Welch’s correction (KIC^AA, n = 3 mice, 13 images; KIC^6-OHDA, n = 3 mice, 15 images). r, P = 0.0014, unpaired t test (KIC^AA, n = 6 mice, 23 images; KIC^6-OHDA, n = 5 mice, 26 images). s P = 0.0002, Mann–Whitney test (KIC^AA, n = 1 mouse, 6 images; KIC^6-OHDA, n = 2 mice, 11 images). Data are represented as median ± SEM. Scale bars = 5 mm (b, c), 500 µm (e, f), 50 µm (h, i), 100 µm (j, k), and 200 µm (l–o). Source data are provided as a Source Data file.