Fig. 4: Most self-renewing EPCR⁺ cells are at the top of a newly defined human CD34⁺ HSPC hierarchy.

a Representative FCM plot illustrating the gating strategy used to sort CB EPCR⁺, CD90⁺EPCR^- and MPP populations and SRC frequency of EPCR⁺ and CD90⁺EPCR^- populations; 95% confidence interval is shown. b Engraftment kinetics of the indicated cell populations. Mice were transplanted with ~1 SRC dose for 1^ry tx and 2^ry tx respectively. Results were from three independent experiments (n = 8–9 mice); median lines are shown. c Representative FCM plots illustrating the phenotype of the denoted sub-populations within CD34⁺CD38^lo/− HSPCs detected at 12 wks in a primary mouse bone marrow transplanted with EPCR⁺ cells. Cumulative data from all the mice that yielded sufficient HSPCs is shown (n = 3–8 mice). The percentages were calculated based on the gates shown (for BMs engrafted with CD90⁺EPCR⁻ cells and MPPs see Supplementary Fig. 3b). d Representative FCM plot showing the presence of EPCR⁺ and CD90⁺EPCR⁻ cells in adult BM (n = 7). e BM derived EPCR⁺ and CD90⁺EPCR⁻ cells were i.v injected into primary NSG recipients with the denoted cell dose (n = 3–7 mice) and human graft was determined 14–16 wks later. Bars are the mean values and error bars are the S.D for the no. of experiments performed. In the LDA experiments the CD201 clone RCR-227 and the CD201 clone RCR-401 antibodies were used, and in the remaining experiments only the latter antibody was used. Source data are provided as a Source Data file.