Fig. 5: Defining the molecular features of EPCR⁺ HSCs.

a Volcano plot representation of the RNA-seq data derived from 5 EPCR⁺ vs 4 CD90⁺EPCR⁻ (top) or vs 5 MPP samples (bottom). b Wikipathways analysis identified significant enriched functional categories (FDR < 0.05 with P-values adjusted using Benjamini–Hochberg method). c Cell division tracking of the denoted populations (n = 4) at day 4 cultured in serum-free condition with a representative FCM analysis of the cells. d Mitochondria membrane potential of the denoted HSPCs determined by TMRE staining (n = 4) with a representative FCM analysis. e ATP production by the denoted cells determined with the CellTiter-Glo^® luminescent assay (n = 3). f Protein synthesis detected in the indicated HSPCs assayed with an OP-Puro protein synthesis kit (n = 3) with a representative FCM analysis. Open and filled histograms represent the signal obtained without (w/o) and with (w) OP-Puro treatment respectively. Normalized values (to the signal derived from EPCR⁺ HSC) are shown. The CD201 clone RCR-401 antibody was used in all the experiments. Bars are the mean values and error bars are the S.D for the no. of experiments performed. Student t-test (two-tailed unpaired in c, e and two-tailed paired in d, f) was used. Source data are provided as a Source Data file.