Table 2 Primers.
From: Human serum triggers antibiotic tolerance in Staphylococcus aureus
Oligonucleotide | Sequence (5′-3′) |
|---|---|
For construction of PvraX-gfp fluorescent reporter plasmid: | |
pCN34_gfp_Fw _vraX | AGCAAAGGAGGTAATATAGGAAAAAAAATGAGTAAAGGAGAAGAACTTTTCACTGG |
pCN34_gfp_Rev_vraX | GTTGTATGCACCGTGATCCAGTGTCATCTGAACCATAGGATCCTCT |
vraX_Fw | TCCTATGGTTCAGATGACACTGGATCACGGTGCATACAACCG |
vraX_rev | CTCCTTTACTCATTTTTTTTCCTATATTACCTCCTTTGCTACTCTATGG |
For construction of PdltA-gfp fluorescent reporter plasmid: | |
pCN34_gfp_Fw_dltA | TCTAATGAGGGAGACTTAATAAAAAAAATGAGTAAAGGAGAAGAAC |
pCN34_gfp_Rev_dltA | AATTATCATCAGCGCAAATAGTGTCATCTGAACCATAGGA |
dltA_Fw | TCCTATGGTTCAGATGACACTATTTGCGCTGATGATAATTCA |
dltA_Rev | TCTCCTTTACTCATTTTTTTTATTAAGTCTCCCTCATTAGAACT |
For construction of PvraUTSR complementation plasmid: | |
pCN34_Fw_vra | TATGTTTTAGAATAGTTACCACAACGTCGTGACTGGGAAA |
pCN34_Rev_vra | TAAGTTTTTAATGACTTTCAAAAACGACGGCCAGTGAATT |
vraUTSR_Fw | AATTCACTGGCCGTCGTTTTTGAAAGTCATTAAAAACTTAACAGG |
vraUTSR_Rev | TTTCCCAGTCACGACGTTGTGGTAACTATTCTAAAACATATGGCA |
For construction of PgraXRS complementation plasmid: | |
pCN34_Fw_gra | AGAAGTTAGATAAAGAACATACAACGTCGTGACTGGGAAA |
pCN34_Rev_gra | AAATGTACCACTCAATAACCAAAACGACGGCCAGTGAATT |
graXRS_Fw | AATTCACTGGCCGTCGTTTTGGTTATTGAGTGGTACATTTGC |
graXRS_Rev | TTTCCCAGTCACGACGTTGTATGTTCTTTATCTAACTTCTGTACC |
For construction of PdltD complementation plasmid: | |
pCN34_Fw_dlt | TATCTTTATAGGCGCCTTTGCGGAAAGAG |
pCN34_Rev_dlt | AGCGCAAATAATTCGCCATTCAGGCTGC |
dltA_pro_Fw | AATGGCGAATTATTTGCGCTGATGATAATTC |
dltA_pro_Rev | TTAATTTCATATTAAGTCTCCCTCATTAGAAC |
dltD_Fw | GAGACTTAATATGAAATTAAAACCTTTTTTACCC |
dltD_Rev | CAAAGGCGCCTATAAAGATATTAAGTTAACAGAACATATTATG |
For construction of Pcls2 complementation plasmid: | |
pCN34_Fw_cls2 | GGCGCCTTTGCGGAAAGAG |
pCN34_Rev_cls2 | ATTCGCCATTCAGGCTGC |
cls2_Fw | AGCCTGAATGGCGAATCTGTTTAACGCCGAACGTG |
cls2_Rev | TTCCGCAAAGGCGCCTTTCGTCAACGGTATCATGAAG |
