Fig. 6: Extensive chromatin remodeling in memory CD8+ T cells is observed upon combined changes in both TCR and IL-2 signaling strength. | Nature Communications

Fig. 6: Extensive chromatin remodeling in memory CD8+ T cells is observed upon combined changes in both TCR and IL-2 signaling strength.

From: T cell receptor and IL-2 signaling strength control memory CD8+ T cell functional fitness via chromatin remodeling

Fig. 6

a Schematic of the comparative analyses conducted between the open chromatin regions (OCRs) in OT-I memory cells (WT, Il2ramut/mut) primed following Lm-N4 versus Lm-T4 infection. b Pie charts show the number of unique OCRs and related genes with unique peaks controlled by changes in TCR, IL-2 or TCR + IL-2 signaling from comparisons depicted in (A). c Venn diagrams show the number of unique or overlapping genes associated with peaks induced by modulating TCR, IL-2 or TCR + IL-2 signals. d Network analysis of biological-process GO term enrichment of the genes with unique peaks induced by changes in TCR, IL-2 or TCR + IL-2 signals. Over-represented GO terms were analyzed by Panther and visualized in a scatterplot. Node color is proportional to the FDR-adjusted p-value of the enrichment. Node size is proportional to the number of genes enriched in each GO term. Enrich in ClusterProfiler was used (over-representation analysis with FDR correction Benjamini and Hochberg. e Heat map shows the transcription factors enriched in unique OCRs induced by modulating TCR, IL-2 or TCR + IL-2 signals. ATAC-seq (n = 3–4 mice) results were summarized from 2 independent replicates of experiments. A linear model was used.

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