Fig. 8: Robust TCR and IL-2 signals induces the most memory CD8+ T cell subset diversity and phenotypes. | Nature Communications

Fig. 8: Robust TCR and IL-2 signals induces the most memory CD8+ T cell subset diversity and phenotypes.

From: T cell receptor and IL-2 signaling strength control memory CD8+ T cell functional fitness via chromatin remodeling

Fig. 8

a FACS analysis of Il2ramut/mut and WT OT-I memory cells primed after infection either Lm-N4 or Lm-T4 ~5 weeks later, after staining with a panel of 26 memory cell-relevant markers. Bar graphs show classically defined effector (TEM), central memory (TCM) subsets or based on cell-surface expression of KLRG1, CD127, CD27 and CX3CR1 (n = 2 mice for Lm-N4 and 3 mice for Lm-T4 in 1 representative of 2 independent replicate experiments). b FlowSOM analysis of each experimental group containing a pool of concatenated OT-I memory cells from 3 mice. Where classically defined TEM and TCM subsets are, is manually circled. c The expression level of indicated markers (Eomes, Bcl-2, TCF-1, CX3CR1) within each node, regulated by each or combined priming signal(s), is represented in a color scale. Data are pooled from 3–4 mice across 2 replicate experiments.

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