Fig. 5: Interactome analysis of SILT FRC and lamina propria ILCs.

a UMAP of sorted CD127⁺ ILCs from Ccl19-EYFP Ltbr^fl/fl and co-housed littermate controls. b ILC composition based on scRNA-seq data. c Heatmap indicating the number of ligand and receptor interactions between SILT FRC and ILCs from Ccl19-EYFP and Ccl19-EYFP Ltbr^fl/fl mice using CellPhone analysis. d Predicted intensity of ligand and receptor interaction between SILT FRCs and ILCs from Ccl19-EYFP and Ccl19-EYFP Ltbr^fl/fl mice based on the CellPhone analysis. The opacity of the circles indicates the degree of statistical significance and the size of the circles provide a measure of the interaction intensity. e Number of RORC⁺ ILC3 residing in SILTs of Ccl19-EYFP and Ccl19-EYFP Il7^fl/fl mice. f Number of CD127⁺ ILCs and ILC subsets from adult Ccl19-Cre Il7^fl/fl mice and co-housed littermate controls. a ScRNA-seq data represents 40,473 CD127⁺ ILCs, n = 6 biological replicates from Ccl19-EYFP Ltbr^fl/fl mice and co-housed littermate controls. e n = 16 and 11 in CP, n = 16 and 12 in imILF, n = 6 and 9 in mILF of 3 mice from two independent experiments in each condition, mean ± SEM. f n = 7 and 7 mice from three independent experiments, mean ± SEM. Statistical analyses were performed using one-way ANOVA with Tukey’s multiple comparison test (e) and unpaired two-tailed Student’s t test (f).