Fig. 6: H&E and Immunofluorescence staining on liver tissues. | Nature Communications

Fig. 6: H&E and Immunofluorescence staining on liver tissues.

From: Protective effect of platinum nano-antioxidant and nitric oxide against hepatic ischemia-reperfusion injury

Fig. 6: H&E and Immunofluorescence staining on liver tissues.

a H&E staining of liver tissues from each group (Untreated, PBS, iNOS, iNOS@ZIF, Pt@ZIF, Pt@ZIF+iNOS@ZIF, Pt-iNOS@ZIF, NAC) after 60 min of ischemia and 12 h of reperfusion. Scale bar: 100 µm. b Immunofluorescence staining of liver tissues with various treatments by using DAPI (blue) for nuclear staining, anti-F4/80 antibody (green) as monocyte/macrophage marker, and anti-CD31 antibody (red) as an endothelial marker from each group. Scale bar: 100 µm. c Enlarged images of immunofluorescence staining with various treatments by using DAPI (blue) for nuclear staining, anti-Ly6G antibody (green) as neutrophil marker and anti-caspase-3 antibody (red) as a cell apoptosis marker. Experiments were performed three times (a–c) with similar results. Scale bar: 50 µm.

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