Fig. 7: Optogenetic inhibition of M2-DMS projections during execution impairs use of recent experience.
a (top left) Schematic and example histology of ArchT optogenetic inhibition of M2-DMS projection neurons. b, c Slice verification of ArchT-mediated inhibition in M2-DMS projection neurons. ** = 2-way RM ANOVA (Current x Light) interaction: F6,6 = 17.0, p = 0.002. d–f Pre-onset inhibition. d %Presses that met criteria, (e) Main effect of inhibition on duration, and (f) Interaction between inhibition and the contribution of the prior duration. g–i As in (d–f) except for inhibition during the duration of the press. In (g), a 2-way RM-ANOVA (Opsin/Fluorophore X Day) revealed a main effect only of Opsin group (F1,9 = 7.59, p = 0.0223), and in (i), a 2-way RM ANOVA (Opsin/Fluorophore x n-back) revealed a main effect only of Opsin group (F1,23602 = 10.5, p = 0.0012). j–l As in (d–f) except for inhibition occurring after press offset. Note that although there is a group difference between ArchT and tdTomato mice in (k), the main effect itself is n.s. in the model for both groups. All tests were two-tailed and multiple comparisons corrected. n0 = Light occurred on press n. n - 1 = Light occurred on press n - 1. tdTomato = tdTomato expressing control mice. ArchT = ArchT expressing mice. In all LME graphs, * without any lines indicate significant terms via F-test on the model (within group comparison), while * with a line indicate significant, between group differences. ***p < 0.001, **p < 0.01, *p < 0.05. n.s. = Not significant. Data are mean + SEM. See also, Source Data.
