Fig. 2: Map-based cloning of barley leaf rust resistance gene Rph3. | Nature Communications

Fig. 2: Map-based cloning of barley leaf rust resistance gene Rph3.

From: The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei

Fig. 2: Map-based cloning of barley leaf rust resistance gene Rph3.

A high-resolution genetic linkage and physical map of the Rph3 locus were constructed based on segregation among 10,411 F2 individuals. Forty-five recombinants were found between flanking markers MLOC_004 and MLOC_023. The Rph3 gene was physically located in an 8519 bp interval based on cv. Barke reference sequence. Two putative genes identified within the window are shown as ORF1 and ORF2. Four independent EMS-induced mutants within the coding sequence of ORF2 indicated that the ORF2 was required for Rph3 resistance.

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