Fig. 6: PDMS sample support design, SLB formation and Snf7 spirals on a soft surface. | Nature Communications

Fig. 6: PDMS sample support design, SLB formation and Snf7 spirals on a soft surface.

From: Snf7 spirals sense and alter membrane curvature

Fig. 6: PDMS sample support design, SLB formation and Snf7 spirals on a soft surface.The alternative text for this image may have been generated using AI.

a Preparation of a soft PDMS sample support for HS-AFM. (1) A PDMS drop is pipetted onto atomically flat mica. (2) The HS-AFM sample stage is deposited on the PDMS droplet. (3) PDMS curing. (4) HS-AFM sample stage with PDMS layer is separated from mica sheet. (5) PDMS surface is O2-plasma treated to enhance hydrophilicity. The PDMS had a Young’s modulus of ~270 kPa (Supplementary Fig. 4). b SLB formation through SUV spreading and fusion (Supplementary Movie 8). c HS-AFM images of slow Snf7 polymerization on a PDMS-SLB (Supplementary Movie 9). d HS-AFM images of rapid Snf7 spiral polymerization on a PDMS-SLB (Supplementary Movie 10). HS-AFM time-lapse of a Snf7 spiral (e) polymerizing into a spiral of increased protrusion height, followed by a collapse into plane and spiral growth, (f) forming a closed stunted Snf7 ring unable of further growth, and (g) polymerizing into a large spiral displaying dynamics of the innermost turn.

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