Fig. 3: KEAP1 regulates FSP1 through NRF2-mediated transcription. | Nature Communications

Fig. 3: KEAP1 regulates FSP1 through NRF2-mediated transcription.

From: A targetable CoQ-FSP1 axis drives ferroptosis- and radiation-resistance in KEAP1 inactive lung cancers

Fig. 3

a Schematic showing ARE binding sites on the FSP1 promoter region. b, c ChIP analyses of NRF2 binding on ARE1 (b) and ARE2 (c) in the FSP1 promoter in A549 cells. d, e ChIP analyses of NRF2 binding on ARE1 (d) and ARE2 (e) in the FSP1 promoter in H1299 KEAP1 KO cells. f Protein levels of KEAP1, NRF2, FSP1 in HEK-293T cells treated with TBHQ. g–j FSP1 promoter-luciferase activities in response to TBHQ treatment (g, h) or in KEAP1 NRF2 DKO HEK-293T cells (i, j). k Protein levels of KEAP1, NRF2, and FSP1 in KEAP1 NRF2 DKO H1299 cells overexpressing FSP1. EV empty vector, OE overexpression. l PI staining to measure cell death upon RSL3 or ML162 treatment in KEAP1 NRF2 DKO H1299 cells overexpressing FSP1. Data were presented as (if mentioned otherwise) mean ± SD; n = 3. P value was determined by unpaired Student’s t-test (3b–e) and two-way ANOVA (3h, i, l); ns not significant. Source data are provided as a Source Data file.

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