Fig. 5: ATAC50 produces functional separation of hGSC cultures. | Nature Communications

Fig. 5: ATAC50 produces functional separation of hGSC cultures.

From: Cell-lineage controlled epigenetic regulation in glioblastoma stem cells determines functionally distinct subgroups and predicts patient survival

Fig. 5

a Consecutive sphere assays comparing ATAC50 clusters. Primary spheres (left), secondary spheres (middle), and tertiary spheres (right). b ELDA comparing ATAC50 clusters. c Frequency of BrdU-positive cells. Data show mean ± SEM. d Collagen invasion assay measuring the invaded area at 24 h. Data show mean ± SEM. a–d Data show mean ± SEM. Two-sided Welch’s t-test was performed on all pair-wise comparisons, and significant differences are indicated with p value. n number of cell cultures. e–g Volcano plots of pair-wise comparisons of AUC scores in ATAC50 C1 (n = 12), C2 (n = 7), and C3 (n = 10) cultures to 28 anticancer drugs. Red circles indicate significantly different drug response. n, number of cell cultures. Two-sided Mann–Whitney was performed on all pair-wise comparisons without multiple adjustment. e ATAC50 C1 versus C2. Red circles in upper left corner, C1 more sensitive. f ATAC50 C1 versus C3. Red circles in upper left corner, C1 more sensitive. g ATAC50 C2 versus C3. Red circles in upper left corner, C2 more sensitive. Red circles in upper right corner, C3 more sensitive. Source data are provided as a Source Data file for Fig. 5a–g.

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