Fig. 2: Engagement of tDNA by the MVV intasome.

a Closeup view of the vDNA-tDNA synapse within the MVV STC. DNA phosphate backbone is shown as cartoon with sugar and bases as sticks. Complementary tDNA strands are in red and pink. The rest of the chains are colored as in Fig. 1a. MVV IN residues involved in the interactions with tDNA are shown as sticks and indicated. b Nucleotide preferences at MVV integration sites. Sequence logos^111,113 represent information content (reported in bits, top) or raw nucleotide frequencies (bottom) at each position within an alignment of 327,911 in vitro MVV intasome integration sites in sheep genomic DNA⁸. Nucleotide positions of the tDNA strand represented by the logos are numbered, and the nucleotide that becomes joined to 3′ vDNA end (corresponding to position 0) is indicated with solid black arrowhead. The dotted arrowhead indicates the insertion position of the second vDNA end into the complementary tDNA strand.