Fig. 5: Application of QS variants for dynamic modular optimization of 4-HC production.
From: Redesigning regulatory components of quorum-sensing system for diverse metabolic control
a Schematic of construction of dynamic control circuits for regulation of 4-HC biosynthetic pathway. b The effect of different triggering OD of QS variants individually driving entC-pchB expression and pqsD-sdgA expression on 4-HC titer. Con represents 4-HC titer generated by the control strain expressing EP and PS modules on constitutive promoters. Gene: entC encodes isochorismate synthase, pchB encodes isochorismate pyruvate lyase, pqsD encodes CoA ligase, sdgA encodes biphenyl synthases. Data shown are mean ± SD (n = 3 independent experiments). Source data are provided as a Source Data file.
