Fig. 3: Extracting scRT from asynchronous human MCF7 cells.
From: Kronos scRT: a uniform framework for single-cell replication timing analysis
a Scatter plots showing the intracellular bin-to-bin variability in function of single-cell ploidy before (left) and after (right) S-phase progression correction. The colour code is the same as in Fig. 1b, c. Kronos scRT can automatically identify the S-phase cells from the asynchronous scWGS data (see Methods). b scRT calculated from unsorted cycling MCF7 breast cancer cells. Same presentation as in Fig. 2a. c Comparison between single-cell and bulk RT data, as in Fig. 2b. d Twidth values calculated for genomic regions classified in five RT categories based on the pseudo-bulk RT values (Very early >0.8, 0.8≥ Early >0.6, 0.6≥ Mid >0.4, 0.4≥ Late >0.2, and Very Late ≤0.2). P values were calculated using the Kronos scRT Compare TW module (“Methods”); * < 0.05, ** < 10−2, *** < 10−3, **** < 10−4.
