Fig. 2: Characterisation of DNA G4 positioning across splicing junctions.

A G4 enrichment for template and non-template strands and stratified by the splicing strength scores of the adjacent splice site. The splicing strength scores for splice junctions with a G4 are significantly lower than for splice junctions without a G4 (Mann–Whitney U, p value <0.001). The splicing strength score bias was found to be dependent on the strand orientation of G4s for splice sites with G4s within 100 nt away (Mann–Whitney U, 3′ss p value <0.05, 5′ss p value <0.001). B Number of consecutive G-runs and relative enrichment at the splicing junction. The error bands in A, B represent 95% confidence intervals from the binomial error.