Table 1 Photoincorporation yields (mole%) into RNAse H Fragments^a.

Target	Experiment	Competing Ligand^b	Mutated site(s)/RNAse Fragment
Target	Experiment	Competing Ligand^b	18S A1195/I: 18S 1151–1206	26S-A3093/II: 26S 3069–3113	26S-A2669;A2672/III: 26S 2638–2702	CrPV-IRES U177;G186 IV: 156–221
Stop-POST5	PAL, 300 µM AzAt	–	1.55 ± 0.20	0.40 ± 0.01	1.07 ± 0.14	3.2 ± 0.8
		eRF1/eRF3/GDPNP	0.07 ± 0.03	0.18 ± 0.10	0.19 ± 0.02	2.9 ± 0.6
		eRF1	0.19 ± 0.07	0.18 ± 0.01	0.20 ± 0.01	–
	PAL, 30 µM AzAt	–	0.26 ± 0.03	0.03 ± 0.01	0.13 ± 0.04	–
		Ataluren, 500 µM	0.06 ± 0.01	0.06 ± 0.01	0.10 ± 0.04	–
		GJ072, 150 µM	0.10 ± 0.01	0.07 ± 0.01	0.07 ± 0.01	–
	PRE, 300 µM AzAt	–	0.012 ± 0.003	0.04 ± 0.01	0.03 ± 0.02	–
80S-IRES	PAL, 300 µM AzAt	–	1.03 ± 0.33	0.50 ± 0.09	1.01 ± 0.12	–
80S-IRES	PAL, 300 µM AzAt	eRF1/eRF3/GDPNP	0.56 ± 0.06	0.55 ± 0.12	0.94 ± 0.23	–

^aError ranges are ± average deviations, n = 2 or 3 (italicized) independent determinations; ^bAdded concentrations: eRF1, 2 µM; eRF3, 4 µM; GDPNP, 1 mM

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