Fig. 2: Cryo-EM analysis of native bovine TG.
From: Formation of thyroid hormone revealed by a cryo-EM structure of native bovine thyroglobulin
a Cryo-EM micrographs of biotinylated TG particles captured on a streptavidin affinity grid. Left, micrograph illustrating particles bound to the streptavidin 2D crystal lattice (5-nm array). Scale bar is at lower left. (N = 34,597). Right, the same image after subtraction of the lattice by Fourier filtering of Bragg spots8. b 2D class averages. c Angular distribution of TG particles. Blue-to-red color gradient marks under-to-over-represented orientations. d FSC curves showing the correlation between the indicated independent half-maps. e Reconstructions of native bovine TG from the indicated data processing procedures. Monomer subunits are colored blue and orange, respectively. N-linked glycosylation sites, red. f Unsharpened density map colored by local resolution (ranges from ~2.7 Å to 8.75 Å).
