Fig. 3: Global reconstruction of native bovine TG and initial density map for hormonogenic site B.
From: Formation of thyroid hormone revealed by a cryo-EM structure of native bovine thyroglobulin
a Upper and middle rows show, respectively, global reconstructions of pre-hormonogenic TG (from ref. 6) and the present analysis of native bovine TG (derived from the combined C2 map) color-coded as in Fig. 1; lower panel shows bovine TG reconstruction in ribbon representation. b FSC curves showing the correlation between the combined C2 density map and the atomic model. c C2 map of bovine TG showing the region encompassing hormonogenic site B (pale red). Sigma, contour level of map. d Detailed view of the density map for site B in native bovine TG. Novel unresolved density extending from the acceptor tyrosine position into an interior pocket is indicated in purple. e Corresponding view of the density map for site B in the pre-hormonogenic structure of human TG6. Acceptor and donor tyrosine densities are indicated in red and blue, respectively.
