Table 1 Cryo-EM and model statistics.
From: Formation of thyroid hormone revealed by a cryo-EM structure of native bovine thyroglobulin
Data collection and processing | Dimer | ASU |
|---|---|---|
Microscope | FEI Krios | FEI Krios |
Mode | EF-TEM Gatan | EF-TEM Gatan |
Energy filter | Gatan GIF Quantum | Gatan GIF Quantum |
Detector | K3 | K3 |
Magnification | 105 kX | 105 kX |
Voltage (kV) | 300 | 300 |
Cumulative dose (e.Å−2) | 54 | 54 |
Defocus range (μm) | 0.5–3.5 | 0.5–3.5 |
Pixel size (Å) | 0.825 | 0.825 |
Refinement symmetry | Combined C1 + C2 | C1 |
Particles | 242813 | 376342 |
Particles for C2-expanded | 485626 | |
Map resolution (Å) | 3.28 | 3.32 |
Map resolution range (Å) | 2.71–10 | 2.79–8.75 |
Sharpening B factor (Å2) | −125.0 | −92.0 |
Sharpening B factor (Å2) for C2-expanded | −101.6 | |
REFINEMENT | ||
PDB ID | 7QTQ | |
EMDB | EMD-14145 | |
Model composition: | ||
Non-hydrogen atoms | 55253 | |
Protein residues | 3577 | |
RMS deviations: | ||
Bond lengths (Å) | 0.007 | |
Bond angles (°) | 0.736 | |
Validation: | ||
Molprobity score (percentile) | 2.08 | |
Clashscore | 9.85 | |
Poor rotamers (%) | 0 | |
Ramachandran plot: | ||
Favored (%) | 89.29 | |
Allowed (%) | 10.71 | |
Disallowed (%) | 0 | |
