Fig. 2: Upregulation of NPTX2 expression in peptidergic DRG neurons under chronic itch-like conditions. | Nature Communications

Fig. 2: Upregulation of NPTX2 expression in peptidergic DRG neurons under chronic itch-like conditions.

From: Neuronal pentraxin 2 is required for facilitating excitatory synaptic inputs onto spinal neurons involved in pruriceptive transmission in a model of chronic itch

Fig. 2: Upregulation of NPTX2 expression in peptidergic DRG neurons under chronic itch-like conditions.The alternative text for this image may have been generated using AI.

a, b Nptx2 mRNA in the cervical DRG and spinal cord (SC) in vehicle- and DCP-treated mice (a: n = 6/group, Mann–Whitney test) and in the cervical DRG in SPF- and CV-NC/Nga mice (b: n = 5/group, unpaired t test). c NPTX2 immunofluorescence in the cervical DRG of vehicle- and DCP-treated mice. Scale bar, 100 μm. d Percentage of NPTX2+ neurons in the cervical DRG and immunofluorescence (IF) intensity of NPTX2 per cervical DRG neurons in vehicle- and DCP-treated mice (n = 4/group, unpaired t test). e Double-immunolabeling of NPTX2 and DRG neuronal markers (CGRP, TRKA, VGLUT2, TRPV1, NF200, and IB4) in the cervical DRG in DCP-treated mice. Scale bar: 100 μm. f Quantitative analysis of the percentage of NPTX2+ neurons colocalized with DRG neuronal markers in DCP-treated mice (CGRP; n = 4, VGLUT2; n = 3, TRPV1; n = 4, NF200; n = 4, and IB4; n = 4). g NPTX2 immunofluorescence in the cervical SDH of vehicle- and DCP-treated mice. In DCP-treated mice, NPTX2 (green) was colocalized with CGRP (red) in the SDH. Scale bar, 200 μm. h Electron microscopy analysis of NPTX2 expression at presynaptic terminal connected to a postsynaptic GRPR+ (mCherry+) neuron in the SDH. NPTX2 and mCherry were visualized by small (white arrowheads) and large (red arrowheads) immunogold particles conjugating antibody for NPTX2 and mCherry, respectively. Yellow arrowhead indicates the postsynaptic density site. Scale bar, 100 nm. Values represent mean ± S.E.M. Source data are provided as a Source Data file.

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