Fig. 1: Tumor-expressed B7x promotes infiltrating Treg populations. | Nature Communications

Fig. 1: Tumor-expressed B7x promotes infiltrating Treg populations.

From: The immune checkpoint B7x expands tumor-infiltrating Tregs and promotes resistance to anti-CTLA-4 therapy

Fig. 1

a Experimental scheme of MC38 tumor engraftment. b Representative flow cytometry analysis of B7x expression in MC38-B7x and MC38-Control cells. c Representative gating strategy for Tregs in CD45+ cells. d, e Populations of CD4+ T cells and CD8+ T cells were analyzed within dissociated tumors (d) and draining lymph nodes (e). f, g Gated on Tregs, surface TGF-LAP and intracellular Ki67 (f), and surface Nrp1 and intracellular Helios (g) was determined. n = 9 mice in control group and 10 mice in B7x group, representative of three independent experiments. For (d–g), error bars represent SEM and P values were calculated by two-tailed Student’s T-test, unadjusted for multiple comparisons.

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