Fig. 2: K⁺ impacts the conformational dynamics of dDAT.

a Differential uptake chart comparing differences in the average deuterium uptake (ΔHDX in deuterons (D)) between the apo (Cs⁺) state to the K⁺ state for the 85 identified peptides at the five sampled time points (orange: 0.25 min; red: 1 min; cyan: 10 min; blue: 1 h; black: 8 h). The individual dDAT peptides are arranged along the x-axis starting from the N-terminal and ending on the C-terminal. The peptide number refers to Supplementary Data Table 3. Positive and negative values indicate reduced and increased HDX, respectively, for the K⁺-containing sample. Structural dDAT motifs are marked along the x-axis. b, c Data from the differential uptake chart exhibiting significant differences (Student’s t-test p-value < 0.01) in deuterium uptake between the apo (Cs⁺) and the K⁺ states mapped onto regions of dDAT on cartoons based on (b) the crystal structure (PDB ID: 4XP1) and (c) a snake diagram. Unchanged and uncovered regions are colored light- and dark grey, respectively. Values represent means (n = 3 independent measurements). The dotted lines ( ± 0.24 D) mark a threshold value for the 95% confidence interval, calculated from the pooled SD for all time points. Data are provided as a Source Data file.