Fig. 5: The designs bind to the human G-CSF receptor.
From: A topological refactoring design strategy yields highly stable granulopoietic proteins
SPR titration sensorgrams of the binding kinetics of A rhG-CSF (Filgrastim), B Boskar3, C Boskar4, and D Boskar4_t2. The binding kinetics analysis (association curve fits shown in spectrum colours) indicates higher apparent binding affinity of inherently bivalent ligands, whereby rhG-CSF and Boskar4_t2 represent two distinct modes of bivalent binding to the G-CSFR.
