Table 1 SPR binding parameters.
From: A topological refactoring design strategy yields highly stable granulopoietic proteins
Analyte | ka (M−1 s−1) | kd (s−1) | Kd (M) |
|---|---|---|---|
rhG-CSF | (3.0 ± 0.3) × 105 | (4.9 ± 2.8) × 10−4 | (1.1 ± 1.6) × 10−9 |
Boskar3 | (3.8 ± 0.4) × 104 | (6.1 ± 0.1) × 10−3 | (1.6 ± 0.2) × 10−7 |
Boskar4 | (4.3 ± 0.3) × 104 | (8.6 ± 0.1) × 10−3 | (2.0 ± 0.2) × 10−7 |
Boskar4_t2 | (3.4 ± 0.5) × 105 | (1.7 ± 0.5) × 10−3 | (5.1 ± 1.9) × 10−9 |
Boskar4_st2 | (5.3 ± 0.7) × 105 | (2.9 ± 1.9) × 10−3 | (5.9 ± 4.4) × 10−9 |
Boskar3_B4 | (4.8 ± 0.7) × 105 | (1.1 ± 0.32) × 10−3 | (2.4 ± 0.9) × 10−9 |
Boskar3_st2 | (4. 9 ± 0.8) × 105 | (2.3 ± 0.39) × 10−3 | (4.8 ± 1.4) × 10−9 |
Boskar3 monomeric | (1.6 ± 0.1) × 105 | (6.3 ± 0.16) × 10−3 | (3.9 ± 2.2) × 10−8 |
Boskar3 dimeric | (1.5 ± 0.2) × 105 | (2.7 ± 0.19) × 10−3 | (1.8 ± 3.0) × 10−8 |
Boskar4 monomeric | (1.4 ± 0.1) × 104 | (1.8 ± 0.32) × 10−2 | (1.2 ± 123.3) × 10−6 |
Boskar4 dimeric | (2.4 ± 0.2) × 105 | (3.7 ± 0.23) × 10−3 | (1.5 ± 1.9) × 10−8 |
