Fig. 3: CycA is recruited by the posterior PCP complex.
From: Cortical Cyclin A controls spindle orientation during asymmetric cell divisions in Drosophila
CycA localization in control (a, d), fz mutant (B) and dsh mutant (c, e). CycA immunoreactivity (red) in prophase pI cells identified by Cut (blue) immunostaining in control (a), fzK21/KD4 (b) and dsh1 (c) pupae 17 h APF. All pI cells are at the same stage as revealed by the γ−tubulin immunostaining (green). Note that the apical-posterior enrichment of CycA (arrow in control, a) is not observed in fzK21/KD4 (b) or dsh1 (c). (d, e) in vivo dynamics of CycA::eGFP (green) in control (d) and dsh1 (e) pupae expressing H2B::RFP (red) under the control of neur to identify pI cells. (f, g) Cortical CycA localization after fz overexpression. CycA (green), Fz (red) and Dsh (blue) immunostaining in pI cell 17 h APF expressing a myc-tagged-Fz form. White arrow points to the apical-posterior CycA crescent whereas yellow arrows indicate a spreading of the cortical recruitment of CycA. n = 20, 14, 10, 20, 16, 8 and 2 in a, b, c, d, e, f and g, respectively. Anterior is to the left. Scale bar, 5 μm.
