Fig. 1: MPRNA for localization using different forms of RNA. | Nature Communications

Fig. 1: MPRNA for localization using different forms of RNA.

From: Context-specific effects of sequence elements on subcellular localization of linear and circular RNAs

Fig. 1

a CircLibA and NucLibA were cloned into four expression vectors; in the 3′ UTR of WT β-globin (spliced) and β-globin-Δintrons (unspliced), and in the middle of circPVT1 or SCRcircPVT1 (circular). b qPCR analysis of nuclear and cytoplasmic fractions following transfection of β-globin plasmids or circular expression vectors. n = 4 biologically independent samples, n = 2 for circular expression vectors. Data are presented as mean values +/− SEM. c Outline of the experimental procedure; libraries were transfected, and cells were fractionated into nuclear and cytoplasmic fractions after 24 h. Libraries for sequencing were generated from total, nuclear and cytoplasmic RNA. d Subcellular localization of the tiles derived from the lncRNA FENDRR and the circRNA circATXN11 in the spliced, unspliced, circPVT1, and SCRcircPVT1 contexts. Color indicates the log2(Nuc/Cyto) of each tile. Cytoplasmic shift is in gray, nuclear shift in green. Source data are provided as a Source data file.

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