Fig. 1: Correlative multimodal imaging for mouse brain tissue samples. | Nature Communications

Fig. 1: Correlative multimodal imaging for mouse brain tissue samples.

From: Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy

Fig. 1: Correlative multimodal imaging for mouse brain tissue samples.The alt text for this image may have been generated using AI.

a Elements of the olfactory sensory pathway in the mouse brain. b Nuclear staining of a 100 µm coronal section of the mouse main olfactory bulb, displaying its histological layers, obtained with widefield fluorescence microscopy. c Diagram of the excitatory wiring of the glomerular columns in the olfactory bulb. d Imaging techniques capable of acquiring soft tissue volumes at the neural circuit scale. e Reconstruction of the LXRT (e1) and SXRT (e2) datasets obtained from the same sample, virtually sliced displaying the glomerular layer. f–h Virtual slices in three regions showing features resolved by each imaging modality in the glomerular layer (f1-2), in a sagittal section (g1-2) and in the mitral cell layer (h1-2). Arrows in (g2) indicate apical dendrites. Source data for (d) are provided as a Source Data file. onl, olfactory nerve layer; gl, glomerular layer; epl, external plexiform layer; mcl, mitral cell layer; ipl, inner plexiform layer; gcl, granule cell layer.

Back to article page