Fig. 7: Multiple SBEM datasets obtained of two regions from the same specimen targeted using correlative ex vivo 2-photon, LXRT, SXRT and SBEM. | Nature Communications

Fig. 7: Multiple SBEM datasets obtained of two regions from the same specimen targeted using correlative ex vivo 2-photon, LXRT, SXRT and SBEM.

From: Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy

Fig. 7: Multiple SBEM datasets obtained of two regions from the same specimen targeted using correlative ex vivo 2-photon, LXRT, SXRT and SBEM.

(a) Reconstruction of an olfactory bulb slab, displaying the regions of interest containing the MOR174/9 and M72 glomeruli in magenta and yellow, respectively. (b–e) Multimodal correlated image data of the same region, displaying the 2p ex vivo dataset of the fixed specimen (b), LXRT (c), SXRT (d) and SBEM (e) of the MOR174/9 (b1–e1) and M72 (b2–e2) regions of interest delineated in (a). f Reconstructions of the SBEM datasets containing both genetically identified glomerular columns.

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