Fig. 2: Identification and analysis of hTSC-specific EGs.
a Overlap of hTSC EGs with core26 and primed hPSC EGs19,20. b Selected pathways that are significantly enriched among hTSC EGs that overlap with core and primed hPSC EGs. c Selected pathways that are significantly enriched among hTSC-specific EGs. d The expression of 63 CTB-enriched hTSC-specific EGs in the CTB, EPI, and PrE of 10 d.p.f. human embryo scRNA-seq data37. e The expression of selected CTB-enriched hTSC-specific EGs in the CTB, EPI, and PrE of human embryo scRNA-seq data37. f–g Live cell counts relative to the mean of control sgRNA-transduced H9 hTSCs 4 (f) or 6 (g) days after seeding. Error bar represents standard error of three biological replicates. Two-tailed student’s t test was used for statistical analysis. “*” indicates a p-value<0.05, “**” indicates a p-value<0.01, and “***” indicates a p-value<0.001. The exact p-values from left to right are 0.000323207, 0.000310896, 0.004363306, 0.000530435, 0.002394918, and 0.005800072 (f). The exact p-values from left to right are 0.000193944, 0.00015954, 0.005236037, 0.00056065, 0.00285678, and 0.048627293 (g). h Phase contrast images of H9 hTSCs transduced with control or targeting sgRNAs at 4 days following seeding. The scale bars indicate 75 μm. The images are representative of three independent replicates in an experiment. i Experimental scheme to assess the requirement of TEAD1 during hTSC derivation and maintenance. j Phase contrast images of H9 WT and TEAD1 KO hTSCs. The scale bars indicate 75 μm. The images are representative of two independent experiments. k Western blot confirming the absence TEAD1 protein in TEAD1 KO hTSCs. The result is representative of two independent experiments. l Differential gene expression analysis between H9 WT and TEAD1 KO hTSCs. WT contains two samples, and TEAD1 KO contains two samples each from three independent clones. m Selected GO biological processes enriched among DEGs significantly upregulated or downregulated in TEAD1 KO hTSCs relative to WT hTSCs. n Selected GO biological processes enriched among DARs significantly more open or closed in TEAD1 KO hTSCs relative to WT hTSCs. o WT and TEAD1 KO hTSC RNA-seq and ATAC-seq data shown in the vicinity of selected genes.
