Fig. 3: Investigation of TEAD1 targets in hTSCs.

a TEAD1 gene expression in published AN and H9 pluripotent and trophoblast cell types¹⁰. b Immunofluorescence staining for TEAD1 in H9 hTSCs. The scale bars indicate 75 μm. The experiment was performed once. c Selected TF binding motifs significantly enriched among TEAD1 CUT&Tag peaks and their p-values according to HOMER Motif Analysis. d TEAD1 CUT&Tag peaks overlapping with naïve hPSC or hTSC ATAC-seq peaks were categorized into three groups: those overlapping with hTSC-specific open chromatin regions (OCRs) (group 1), shared OCRs (group 2), or naïve hPSC-specific OCRs (group 3). e Top GO biological processes significantly enriched among group 1 (blue) and group 2 (gray) TEAD1 CUT&Tag peaks and their p-values. f The AN and H9 hTSC ATAC-seq signal over group 1-3 TEAD1 CUT&Tag peaks¹⁰. g The AN and H9 naïve hPSC ATAC-seq signal over groups 1–3 TEAD1 CUT&Tag peaks¹⁰. h CUT&Tag and hTSC ATAC-seq signal of group 1-3 TEAD1 CUT&Tag peaks. i Expression of genes in H9 hTSCs¹⁰, binned by the distance of TEAD1 CUT&Tag peaks to TSS. Two independent samples were used for analysis. Boxplot presents the 25th, median, and 75th quartiles, the whiskers extend 1.5 of interquartile ranges, and the dots are outside values >1.5 times and <3 times the interquartile range beyond either end of the box. j–l Percentage of all genes, all TEAD1 target genes, group 1 TEAD1 target genes, and group 2 (excluding those already in group 1) TEAD1 target genes that are significantly up- or downregulated in AN and H9 hTSCs vs. naïve hPSCs (j), hTSCs vs. STBs (k), hTSCs vs EVTs (l)¹⁰. m TEAD1 CUT&Tag and WT and TEAD1 KO hTSC RNA-seq data shown in the vicinity of selected genes. n TEAD1 CUT&Tag and WT and TEAD1 KO hTSC ATAC-seq data shown in the vicinity of selected genes.