Fig. 1: Genome complexity and sterility of domesticated S. cerevisiae.
From: Unlocking the functional potential of polyploid yeasts
a Polyploid strains are characterised by common genomic hallmarks (bottom box), which are detected in both strains used in this study. b Schematic depicting the RTG framework: we initially quantify and optimise the meiotic progression, followed by selection of novel colony phenotypes. Finally, RTG samples are sequenced and phenotyped to identify clones with improved industrial traits. c Left: level of heterozygosity (number of heterozygous markers/kbp) across chromosomes, total number of heterozygous markers n = 40431 OS1364 (green), n = 39376 OS1431 (purple). Right: Heterozygous markers partitioned according to the frequency of the non-reference allele. d Meiotic progression measured by nuclei DAPI staining, reported as the average (n = 3 replicates) of the percentage of cells that have progressed over the first (MI) and second (MII) meiotic division. The error bars represent the standard deviation. On the right, bar plot representing the spore viability (three viable spores/400 OS1364, one viable spore/400 OS1431). The top arrow is a qualitative description of the meiotic progression based on the results of the DAPI staining. e The bar plot represents the number of high-impact variants affecting essential (red) or non-essential (grey) genes and further divided into homozygous (Hom) if present in all the haplotypes, and heterozygous (Het) if not present in all the haplotypes. Source data are provided as a Source Data file.
