Table 1 Turnover of HTER variants, labelled with tetramethyl-rhodamine Halotag ligand (TMR, 250 nM, 6 h), following the label removal, the samples were chase-imaged in the absence or presence of an ER stressor, tunicamycin (Tm, 0.5 µg/mL).

From: Stress-induced protein disaggregation in the endoplasmic reticulum catalysed by BiP

HTER variant

Untreated t1/2 (hours)

t1/2 in Tm-induced stress (hours)

WT

41 ± 9

174 ± 21

K73T

32 ± 3

58 ± 7

L172Q

7 ± 2

27 ± 5

M21KF86L

28 ± 11

15 ± 3

  1. The time of the decrease of TMR fluorescence to 50% of the initial intensity (half-life, t1/2) was calculated from triplicates by fitting the curves to an exponential decay function (Supplementary Fig. 8, see Methods for details).
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