Fig. 1: MYC induces a profound transcriptional reprogramming in murine prostate lobes. | Nature Communications

Fig. 1: MYC induces a profound transcriptional reprogramming in murine prostate lobes.

From: MYC drives aggressive prostate cancer by disrupting transcriptional pause release at androgen receptor targets

Fig. 1

a Graphical summary of the experimental design. b, c Transcriptional profiling of WT and MYC-transformed VP reveal high concordance for the total number of genes detected (b) and their expression levels (c) between bulk and single-cell RNA-seq (VP; matched bulk and single-cell RNA-seq; n = 1 per genotype). d, e Sample-sample correlation (d) and principal component analysis (e) between bulk and matched single-cell transcriptome identifies distinct transcriptional profiles across murine prostate lobes (AP, DLP, VP; matched bulk and single-cell RNA-seq; n = 1 per genotype). f Single-cell census of WT and MYC-transformed AP, DLP and VP. tSNE of scRNA-seq profiles colored using known markers identified nine major subpopulations across prostate lobes (AP, DLP, VP; n = 1 per genotype). g–i The human MYC transgene (hg19MYC) expression is largely restricted to the luminal compartment (g AP, DLP, VP; n = 1 per genotype) and predominantly expressed in the VP (h Source data are provided as a Source Data file), in accordance with the penetrance of prostatic intraepithelial neoplasia (i PIN; n = biologically independent animals; mean ± SD; Source data are provided as a Source Data file17). WT: wild-type; VP: ventral prostate; DLP: dorsolateral prostate; AP: anterior prostate.

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