Fig. 3: Biotransformation probing the function of SdnB and SdnH.

a Biotransformation of 2 by A.nidulans expressing SdnB. b Biotransformation of 2 by A.nidulans expressing SdnH. c Biotransformation of 8 by A.nidulans expressing SdnB. d Biotransformation of 4 by A.nidulans expressing SdnH. Compound 4 cannot be desaturated by SdnH to form 7 (and then to 5) but instead was reduced to 3 by endogenous reductases in A. nidulans. The chromatograms in all cases are extracted from mass spectra of the base peak for each compound.