Fig. 6: SdnG catalyzes efficient in vivo cyclization of 10 but not 7. | Nature Communications

Fig. 6: SdnG catalyzes efficient in vivo cyclization of 10 but not 7.

From: Discovery and characterization of a terpene biosynthetic pathway featuring a norbornene-forming Diels-Alderase

Fig. 6

a Metabolic profiles of A. nidulans transformed with SdnA-C-B-H-F in the absence and presence of SdnG. b Metabolic profiles of A. nidulans transformed with SdnA-C-B-H in the absence and presence of SdnG. The cyclization of 7 is not fast enough to compete with shunt redox pathways (to form 5 and 6) and only trace amounts of 9 is formed regardless the absence or presence of SdnG. The chromatograms in all cases are extracted from mass spectra of the base peak for each compound.

Back to article page