Fig. 1: MRNIP formed puncta in tumor cells and its high expression was associated with the radioresistance and poor prognosis of CRC patients. | Nature Communications

Fig. 1: MRNIP formed puncta in tumor cells and its high expression was associated with the radioresistance and poor prognosis of CRC patients.

From: MRNIP condensates promote DNA double-strand break sensing and end resection

Fig. 1: MRNIP formed puncta in tumor cells and its high expression was associated with the radioresistance and poor prognosis of CRC patients.The alternative text for this image may have been generated using AI.

a Screening of DNA damage repair-related proteins that undergo LLPS. Details could be found in Supplementary Figure 1a. b MRNIP-GFP showed puncta in the nucleus of HEK 293 T cells. HEK 293 T cells were transfected with plasmid for 24 h before observation. c Immunofluorescence of endogenous MRNIP in HCT8 and HeLa cells. d Immunofluorescence assay showing that MRNIP formed puncta in 3 out of 6 CRC tissues. Frozen sections of CRC tissues were analyzed using Immunofluorescence assay. e MRNIP protein was upregulated in CRC tissues. Twenty-seven CRC tissues and adjacent normal tissues were analyzed. f, g Higher MRNIP expression was correlated with shorter survival time of CRC patients. The correlations were analyzed with Kaplan-Meier curve and Log-rank test. h CRC patients with higher MRNIP level were more resistant to radiotherapy. TRG, Tumor regression grade. For (f–h), CRC tissues from 213 patients were analyzed. i, j Xenograft model showed that MRNIP depletion sensitized tumor cells to radiation. For (j), each line represents one xenograft.

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