Fig. 6: HDLBP crosslinks to tRNAs decoding CU/UU-containing codons. | Nature Communications

Fig. 6: HDLBP crosslinks to tRNAs decoding CU/UU-containing codons.

From: HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins

Fig. 6: HDLBP crosslinks to tRNAs decoding CU/UU-containing codons.The alternative text for this image may have been generated using AI.

a Difference in codon frequencies in the P-site (top) and E-site codons (bottom) in HDLBP KO vs. WT. Mean codon shift was calculated for four replicates (mean ± standard deviation is shown). b Enrichment of tRNAs in HDLBP PAR-CLIP and their binding sites. T-C transitions in tRNAs were normalized to total tRNA abundance and ranked from highest to the lowest value (left to right). For each T-C transition, we displayed its transition specificity (T-C transition vs. total read coverage). Mean values of two PAR-CLIP biological replicates ± SD are depicted. Total log2-transformed tRNA abundance and codon usage are also shown (top). c (Left) Browser representation of alignment to tRNA Leu-UAG. T-C transitions in the D-loop and V-region are indicated for the HDLBP PAR-CLIP dataset. The second track shows coverage in the total RNA sample. (Right) HDLBP crosslinked uridines are indicated with respect to secondary tRNA structure.

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