Fig. 1: Lethality of Kdm5cΔ/Δ female embryos.
From: Activation of Xist by an evolutionarily conserved function of KDM5C demethylase
a, b Relative quantification of Kdm5c RNA levels in wild-type XX female and XY male EpiSCs (a) and ESCs (b) by RT-qPCR. Each bar in the graph represents an individual cell line analyzed by RT-qPCR in triplicate. Data are presented as mean \(\pm\) standard deviation between technical replicates. c–f Quantification of liveborn mice (c–e) and recovery of embryonic day (E) 5.5 and 6.5 embryos (f). g Representative micrographs of E5.5 embryos. 13 litters were analyzed with similar results. Scale bars, 50 μm. Expression of a paternally-inherited X-Gfp transgene marks female embryonic epiblasts (Epi) but not extraembryonic ectoderm (EE) or visceral endoderm (VE) due to random X-inactivation in the epiblast lineage and imprinted X-inactivation of the paternal X-chromosome in extraembryonic lineages60,61. See also Supplementary Fig. 1a. Source data are provided as a Source Data file.
