Fig. 5: KDM5C upregulation of Xist expression via putative enhancer activation.
From: Activation of Xist by an evolutionarily conserved function of KDM5C demethylase
a KDM5C enrichment at the Xist locus detected by ChIP-Seq. See Supplementary Table 7 for ChIP-seq read quantification. b ChIP-qPCR quantification of H3K4me1, H3K4me2, H3K4me3, and H3K27ac enrichment at the Xist locus. Locations of PCR amplicons are denoted beneath the browser views in (a). ChIPs were performed on day 2 differentiated EpiLCs generated from two independent ESC lines/genotype. qPCRs were performed in triplicate for each ChIP sample. Data are presented as mean \(\pm\) SEM between ChIP replicates. Selected pairwise comparisons are shown. *P ≤ 0.05; **P < 0.01; ***P < 0.001, Welch’s two-sided t tests. See Supplementary Table 8 for all pairwise statistical comparisons. Source data are provided as a Source Data file.
