Fig. 3: Zinc and ASD-linked mutations alter CTTNBP2 mobility in COS1 cells. | Nature Communications

Fig. 3: Zinc and ASD-linked mutations alter CTTNBP2 mobility in COS1 cells.

From: Phase separation and zinc-induced transition modulate synaptic distribution and association of autism-linked CTTNBP2 and SHANK3

Fig. 3: Zinc and ASD-linked mutations alter CTTNBP2 mobility in COS1 cells.

a WT GFP-CTTNBP2. b GFP-CTTNBP2 D570Y mutant. c GFP-CTTNBP2 M120I mutant. FRAP was performed to analyze the protein mobility of GFP-tagged WT and mutant CTTNBP2, as indicated, in COS1 cells. ZnSO4 or CuSO4 (1 mM) was added to culture medium 30 min before photobleaching as indicated to assess their effects. Black arrow indicates the direction of the live-imaging time-series. Normalized fluorescence intensity and the immobile fraction are shown in the plots at right. For immobile fraction plots, a Kruskal–Wallis test with Dunn’s multiple comparisons test was conducted for a and c, and a one-way ANOVA with Bonferroni’s multiple comparisons test was conducted for b. All statistical analysis and results are summarized in the Statistic Information in the source data file. ns., non-significant. Scale bar: 1 μm.

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