Fig. 2: TP53 WT PQL cells retain tumor-initiating capacity in vitro and in vivo. | Nature Communications

Fig. 2: TP53 WT PQL cells retain tumor-initiating capacity in vitro and in vivo.

From: p53 wild-type colorectal cancer cells that express a fetal gene signature are associated with metastasis and poor prognosis

Fig. 2: TP53 WT PQL cells retain tumor-initiating capacity in vitro and in vivo.

A–C Number of PDOs (upper panels) and diameter (lower panels) of TP53 WT PDO5 and PDO66 A, TP53 mutant PDO4 and PDO8 B, and TP53 KO PDO5 C treated with 5-FU + Iri. as indicated, and left for 2 weeks with fresh medium. 300 cells/well were seeded (n = 6 wells examined for TICs and n = more than 50 spheres examined whenever possible for diameters, from three biologically independent experiments). D Bioluminescence images of NGS mice after intracardiac injection of 40,000 luciferase-PDO5 CT and IC20 or IC30-treated cells (from 1 out of 2 biologically independent experiments). E Percentage of healthy and metastasis-carrying mice at week 15 (n = 14 (Untreat.), 6 (IC20), and 11 (IC30) mice examined over two biologically independent experiments). F Relative photon flux measurement of metastasis initiation in mice injected with PDO5 CT and IC30-treated cells (n = 6 (Untreat.) and 5 (IC30) mice examined from one experiment). G–I Total tumor weight of in situ tumors and intraperitoneal implants per animal in the different experimental groups G (n = 5 mice examined over two biologically independent experiments), number of intraperitoneal implants H (n = 3 mice examined from 1 experiment), and photographs of tumors derived from orthotopically implanted CT, IC20 and IC30-pre-treated PDOs in nude mice I. J, K IHC analysis of ki67 in in situ tumors and implants J and percentage of ki67+ cells in the indicated conditions K (n = more than four independent regions examined). L Tumor weight of intraperitoneal implants of tumors derived from orthotopically implanted CT and IC30-pre-treated PDO5, PDO8 and PDO4 in nude mice (n = 2 (Untreat.) and 5 (IC30) mice examined from 1 experiment). For all applicable figure panels, data are mean ± SD. Significance (p) was calculated with one-way ANOVA test, except for C (two-sided Student’s T test), E (two-sided logistic regression trend test) and F and G (two-way ANOVA test). ****p < 0.0001; n.s., no significant. 5-FU, 5-fluorouracil; Iri, irinotecan. Source data are provided as a Source Data file.

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