Fig. 6: Identification of a fetal ISC signature with prognosis value in CRC.

A Unsupervised hierarchical cluster analysis of patients according to 28up + 8down-feISC gene signature leading to the classification of patients into four subsets (colored in red, green, light blue, and purple). Tumor staging is indicated. B Kaplan–Meier representation of disease-free survival (DFS) over time for patients according to 28up + 8down-feISC signature selected according to A for Marisa (28up = high/8down = low n = 66, 28up = low/8down = high n = 114 and unclassified n = 386), Jorissen (28up = high/8down = low n = 114 and 28up = low/8down = high n = 112) and TCGA (28up = high/8down = low n = 39, 28up = low/8down = high n = 96 and unclassified n = 194) colorectal cancer databases. Patients were selected according to cluster analysis of the 28up + 8down-feISC signature. C–E Kaplan–Meier curves representing patients DFS classified according to cluster analysis of the 28up + 8down-feISC signature of stage II (n = 149) C, stage II and III (n = 468) D, and stage IV (n = 60) E patients from Marisa database. F Kaplan–Meier representation of patients DFS classified according to TP53 in the Marisa data set (TP53 WT n = 161 and TP53 MUT n = 190). G Kaplan–Meier representation of DFS of patients from the Marisa data set classified according to cluster analysis of the 28up + 8down-feISC signature in TP53 WT (n = 85) and TP53 mutant (n = 20) groups. H Kaplan–Meier representation of DFS in CMS4³⁵ patients from Marisa cohort classified based on 28up + 8down-feISC signature (n = 91). Data in A show normalized, centered, and scaled Illumina probe set intensities on a log₂ scale. The stage lane represents the subtype corresponding to each patient. We used Cox proportional hazards models for statistical Kaplan–Meier analysis and log-rank two-sided p value (see Supplementary Table S3). HR, hazard ratio.