Fig. 2: TUBB4A KO reduces proliferation and migration of prostate cancer cells. | Nature Communications

Fig. 2: TUBB4A KO reduces proliferation and migration of prostate cancer cells.

From: TUBB4A interacts with MYH9 to protect the nucleus during cell migration and promotes prostate cancer via GSK3β/β-catenin signalling

Fig. 2

A–D Confirmation of TUBB4A KO, cell growth, clone formation, and statistical analysis of PC3 cells. Cell growth was measured by live cell kinetic imaging with walk-away automation using a Lionheart™ FX cell imager. A colony was considered to be 50 cells or more as determined microscopically. E confirmation of TUBB4A KO and rescue of DU145 cells. F–H Cell growth, clone formation, and statistical analysis of DU145 cells. I, J Confirmation of TUBB4A knockdown (KD) and cell growth of LNCaP cells. K Cell growth assay of LNCaP cells transfected with TUBB4A siRNAs in androgen-depleted culture medium. L, M 3D soft-agar colony formation and statistical analysis of PC3 cells. A 3D colony was counted based on the capacity of single cells to grow to colonies consisting of at least 50 cells. Scale bar, 500 μm. N, O Transwell assay and statistical analysis of PC3 cells. Scale bar, 100 μm. P, Q 3D soft-agar colony formation and statistical analysis of DU145 cells. Scale bar, 500 μm. R, S Transwell assay and statistical analysis of DU145 cells. Scale bar, 100 μm. Data are replicated 3 (J, K, M, Q), 4 (B, F), 6 (D, H), and 10 (O, S) times. Data are presented as the means and SD with a repeated measures ANOVA (B, F, J, K) or an ANOVA followed by Tukey’s post hoc t test (D, H, M, O, Q, S). Source data are provided as a Source data file. Scr, scramble; KO, knockout; NC, negative control siRNA; siRNA, small interfering RNA; NS, no significant difference.

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