Fig. 4: TUBB4A interacts with MYH9 and their relevant DNA damage response and signaling pathways in prostate cancer cells. | Nature Communications

Fig. 4: TUBB4A interacts with MYH9 and their relevant DNA damage response and signaling pathways in prostate cancer cells.

From: TUBB4A interacts with MYH9 to protect the nucleus during cell migration and promotes prostate cancer via GSK3β/β-catenin signalling

Fig. 4

A Bands on an SDS-PAGE gel after TUBB4A protein pull-down in DU145 cells. Red and blue arrows indicate the bands presumed to be MYH9 and TUBB4A, respectively, in the pulldown. B TUBB4A-interacting proteins as determined by mass spectrometry analysis. Red arrow indicates MYH9 and its potential site on SDS-PAGE gel. C, D Co-immunoprecipitation (IP) assay of TUBB4A and MYH9. The input was diluted as 1/10. E Co-localization of TUBB4A and MYH9 by IF staining. Scale bar, 100 μm. F Co-localization of TUBB4A and MYH9 at the forefront of the nucleus during migration in 3D collagen gels determined by IF staining. Scale bar, 10 μm. G, H Western blotting showing changes in proteins of DNA damage response and proteins of the NF-κB p65 and IKK complex in DU145 and PC3 cells with or without TUBB4A and MYH9. I Western blotting showing changes in proteins of DNA damage response and proteins of the intrinsic apoptosis pathway in DU145 cells with or without TUBB4A and MYH9. J Western blotting showing changes in proteins of NF-kB-relevant signaling pathways in DU145 cells with or without TUBB4A and MYH9. Source data are provided as a Source data file. Scr, scramble; KO, knockout; NC, negative control siRNA; siRNA, small interfering RNA; NS, no significant difference.

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