Fig. 5: TUBB4A/MYH9-regulated GSK3β/β-catenin signaling pathway in prostate cancer cells. | Nature Communications

Fig. 5: TUBB4A/MYH9-regulated GSK3β/β-catenin signaling pathway in prostate cancer cells.

From: TUBB4A interacts with MYH9 to protect the nucleus during cell migration and promotes prostate cancer via GSK3β/β-catenin signalling

Fig. 5

A, B Co-IP assay of TUBB4A and MYH9 in DU145 cells. The input was diluted at 1/10. C, D Co-IP assay of TUBB4A and N-terminal (N-MYH9) or C-terminal (C-MYH9) of MYH9-Flag in DU145 cells. E Western blotting for changes in proteins of the GSK3β/β-catenin signaling pathway and EMT markers in DU145 cells with or without TUBB4A and MYH9. F Western blotting showing the effect of TUBB4A KO on MYH9 and GSK3β stability in DU145 cells incubated with cycloheximide (CHX) at the indicated time points. G Ubiquitination of GSK3β in DU145 cells with or without TUBB4A and MYH9. Cells were either untreated or treated with proteasome inhibitor MG132 (10 μM) for 12 h. Equal aliquots of the cellular lysates were immunoprecipitated with anti-GSK3β antibodies. Source data are provided as a Source data file. Scr, scramble; KO, knockout; NC, negative control siRNA; siRNA, small interfering RNA.

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