Fig. 7: TUBB4A KO decreases tumor metastasis in the lungs of NSG mice. | Nature Communications

Fig. 7: TUBB4A KO decreases tumor metastasis in the lungs of NSG mice.

From: TUBB4A interacts with MYH9 to protect the nucleus during cell migration and promotes prostate cancer via GSK3β/β-catenin signalling

Fig. 7

A Schematic diagram of S.C. xenograft tumors followed for up to 50 days in NSG mice. B Luciferase imaging of xenograft tumors and metastases after implantation. C, D Statistical analysis of tumor nodules and burdens of lung metastasis at day 50. E H/E and IF staining of metastatic tumors. Scale bar, 100 μm. F IHC staining of c-MYC, p-IKKα/β, and NF-κB (p-p65) of lung metastatic tumors in DU145 xenografts. Scale bar, 100 μm. G Schematic diagram of S.C. xenograft tumors followed for up to 90 days in NSG mice. H Representative tumor metastases in the lung at day 50. Scale bar, 500 μm. I, J Statistical analysis of tumor nodules and burdens of lung metastasis at day 50. K IHC staining of TUBB4A and E-cadherin in scrambled/KO mixed S.C. xenografts. Scale bar, 50 μm. L IHC staining of TUBB4A in scrambled/KO mixed lung metastatic tumors at day 90. Scale bar, 100 μm or 500 μm. M Statistical analysis of tumor nodules of lung metastasis at day 90. Data are replicated 10 times (C, D, I, J, M). Data are presented as the medians and interquartile ranges with a Mann–Whitney U (C, D) or Kruskal–Wallis followed by a Benjamini–Hochberg false discovery rate test for multiple comparisons (I, J). Data are presented as the means and SD with a two-tailed t test (M). Source data are provided as a Source data file. Scr, scramble; KO, knockout; s.c., subcutaneous injection; luc, luciferase.

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