Fig. 1: bmp4 is required for regeneration initiation.
From: The planarian wound epidermis gene equinox is required for blastema formation in regeneration
a bmp4 RNAi animals do not regenerate. n = 6 (left) and n = 2 (right and bottom) independent experiments. Dotted yellow line, amputation plane. b Heatmap shows expression of wound-induced genes, neoblast markers, anterior and posterior PCGs after bmp4 RNAi. Some posterior-PCG labeled genes have not been connected to a signaling pathway but are regionally expressed in muscle. c Graphs show decreased expression of wound-induced genes and neoblast-specific genes in bmp4 RNAi animals. d Reduced wound-induced gene expression, e reduced neoblast accumulation (smedwi-1) and expression of anterior markers (ndl-5) after bmp4 RNAi. f Failed posterior PCG rescaling (wntP-2) in bmp4 RNAi animals. Blue arrow, anterior expression edge of wntP-2. g Volcano plot shows dd_20318 expression is significantly lower in bmp4 RNAi animals than in controls. h Reduced dd_20318 expression at 18 hpa in bmp4 RNAi animals. Results shown in d–f, h are from two independent experiments. Colored boxes, area depicted in pictures. Scale bars, 100 μm.
