Fig. 5: equinox is required for PCG rescaling and neoblast proliferation at wounds.
From: The planarian wound epidermis gene equinox is required for blastema formation in regeneration
a Heatmap shows reduced expression of neoblast markers and anterior PCGs and no change of posterior PCG expression at anterior-facing wounds after equinox RNAi. b Reduced expression of neoblast-specific genes (left) and reduced numbers of mitotic cells (right) at equinox RNAi anterior-facing wounds. For phosphorylated histone H3 (H3P) labelings, n = 6 independent animals for control 0 hpa, n = 7 for control 6 hpa, n = 12 for control 48 hpa, and n = 5 for control 96 hpa; n = 6 independent animals for equinox RNAi 0 hpa, n = 9 for equinox RNAi 6 hpa, n = 12 for equinox RNAi 48 hpa, and n = 6 for equinox RNAi 96 hpa over two independent experiments. Data are presented as mean ± SD and analyzed with unpaired two-tailed Student’s t test. c No neoblast wound accumulation or rescaling of posterior PCG expression (wntP-2) after equinox RNAi. n = 3 independent experiments. Blue arrows, anterior edge of PCG expression. (d) No anterior PCG expression (ndl-2, ndl-5, ndl-4, sFRP-1) or anterior pole formation (notum), and asymmetric posterior pole formation (wnt1 and wnt11-2) after equinox RNAi. Results shown are representative from at least two independent experiments. Blue arrows, midline. Colored boxes, area depicted in pictures. Source data are provided as a Source Data file. Scale bars, 100 μm.
